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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: J Physiol. 2020 Jun 13;598(15):3085–3105. doi: 10.1113/JP279423

Fig 4. Representative Magpix analysis of HIE supernatants after stimulation by host and microbial factors showing robust cytokine production of IL-8, GRO, MCP-1 and IL-1RA.

Fig 4.

Heat map representation of high cytokine concentrations in the supernatant after enteroids were stimulated with host cytokines TNF or IL-1α (1 μg/ml)(A) or microbial stimuli LPS, LTA, or flagellin (1 μg/ml)(B) in simplified CMGF− media for 16 hrs. Representative individual Magpix results comparing the amount of cytokine production between the stimulated group and simplified CMGF− media control group for C. IL-8, D. GRO, E. MCP-1, and F. IL-1RA. One Way ANOVA * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001. n=3 wells/experiments, representative of 3 independent experiments.