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. 2020 Nov 5;8:590008. doi: 10.3389/fcell.2020.590008

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Copyright © 2020 Ge, Yang, Li, Wang, Lin, Feng, Chen, Jiang, Wang, Hu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cortical neuron identity for astrocyte-converted neurons in monkey cortex. (A–B) Representative images showing triple immunostaining of mCherry (red), SV2 (green) and NeuroD1 (white) in the NeuroD1-mCherry or mCherry-infected areas at 2- or 6-months post viral injection (21 days after stroke). Data shows significantly increased synaptic puncta (SV2) in the NeuroD1-infected areas, compared to the control mCherry-infected areas. Note a significant decrease of NeuroD1 expression at 6 months following viral infection. Scale bars, 50 μm. (C,D) Representative images showing triple immunostaining of mCherry (red), Tbr1 (green) and NeuroD1 (white) in the NeuroD1-mCherry or mCherry-infected areas at 2- or 6-months post viral injection (21 days after stroke). Most of the NeuroD1-expressing neurons were Tbr1⁺. Scar bars, 50 μm.