. 2020 Nov 5;11:546230. doi: 10.3389/fmicb.2020.546230

TABLE 1.

Fermentation parameters and DGLA production of the shake-flask cultures of the engineered A. oryzae using different nitrogen sources.

Parameters	Nitrogen source
	Yeast extract	KNO₃	NaNO₃	(NH₄)₂HPO₄	NH₄Cl	(NH₄)₂SO₄
X_m (g/L)	16.87 ± 1.05^c	23.90 ± 1.10^b	24.75 ± 1.32^a	11.92 ± 1.20^e	12.86 ± 1.11^d	13.18 ± 0.64^d
μ_max (day^–1)	0.28 ± 0.01^c	0.36 ± 0.03^b	0.43 ± 0.02^a	0.18 ± 0.00^e	0.23 ± 0.02^d	0.29 ± 0.01^d
Y_X/S (g_X/g_S)	0.28 ± 0.01^c	0.39 ± 0.02^b	0.41 ± 0.02^a	0.19 ± 0.00^e	0.21 ± 0.01^de	0.22 ± 0.02^d
Qx (g_X/L.h)	0.31 ± 0.02^b	0.50 ± 0.03^a	0.52 ± 0.02^a	0.22 ± 0.03^b	0.24 ± 0.01^b	0.24 ± 0.01^b
DGLA titer (mg/L)	92.78 ± 1.24^c	100.38 ± 1.32^b	116.32 ± 2.08^a	35.35 ± 2.05^f	45.42 ± 1.17^e	55.35 ± 2.67^d
GLA titer (mg/L)	230.91 ± 2.75^c	263.90 ± 2.83^b	284.62 ± 3.88^a	101.83 ± 0.38^e	127.70 ± 1.69^d	139.70 ± 1.39^d
Lipid titer (mg/L)	2946.27 ± 15.32^b	2820.44 ± 34.32^b	3193.01 ± 37.89^a	495.36 ± 13.88^c	294.32 ± 11.88^d	396.78 ± 3.51^cd
DGLA content (% in DCW)	0.55 ± 0.09^a	0.42 ± 0.04^c	0.47 ± 0.05^b	0.29 ± 0.02^e	0.35 ± 0.02^d	0.42 ± 0.03^c
GLA content (% in DCW)	1.36 ± 0.11^a	1.10 ± 0.01^bc	1.15 ± 0.01^b	0.85 ± 0.03^e	0.99 ± 0.03^d	1.06 ± 0.02^cd
Lipid content (% in DCW)	19.60 ± 0.00^a	11.80 ± 0.00^b	12.90 ± 0.14^b	4.80 ± 0.00^c	2.60 ± 0.57^d	3.40 ± 0.28^cd

All data are presented as mean values with SD. Values marked with different superscript letters in the same row are significantly different (p < 0.05).