. 2020 Nov 5;11:546230. doi: 10.3389/fmicb.2020.546230

TABLE 4.

Fermentation parameters and DGLA production of the engineered A. oryzae cultures grown in a stirred-tank bioreactor using the CSH medium (0% ML) and CSH medium supplemented with different ML amounts (the culture pH was constantly controlled at 6.0).

Parameters	ML amount (% v/v)
	0.0	0.5	1.0	3.0	5.0
X_m (g/L)	13.92 ± 1.04^c	15.41 ± 1.49^bc	16.67 ± 1.30^b	19.41 ± 0.78^a	20.23 ± 0.45^a
μ_max (day^–1)	0.19 ± 0.03^c	0.21 ± 0.02^bc	0.22 ± 0.02^abc	0.26 ± 0.01^ab	0.27 ± 0.01^a
Y_X/S (g_X/g_S)	0.23 ± 0.01^c	0.25 ± 0.01^bc	0.28 ± 0.02^abc	0.32 ± 0.01^ab	0.33 ± 0.01^a
Q_X (g_X/L.h)	0.19 ± 0.01^b	0.23 ± 0.02^ab	0.26 ± 0.01^ab	0.30 ± 0.02^a	0.31 ± 0.02^a
DGLA titer (mg/L)	48.61 ± 2.55^d	123.30 ± 2.48^b	170.43 ± 1.55^a	121.24 ± 1.21^b	111.75 ± 1.86^c
GLA titer (mg/L)	52.83 ± 2.58^e	117.10 ± 2.28^d	217.34 ± 4.21^a	184.50 ± 2.60^b	178.10 ± 5.33^c
Lipid titer (mg/L)	626.40 ± 33.22^c	838.38 ± 19.44^b	1085.36 ± 20.44^a	955.55 ± 16.55^ab	920.30 ± 50.06^b
Y_DGLA/X (mg/g_X)	4.45 ± 0.21^d	8.00 ± 0.33^b	10.23 ± 0.65^a	6.24 ± 0.11^c	5.52 ± 0.05^cd
Y_GLA/X (mg/g_X)	4.83 ± 0.17^d	7.60 ± 1.02^c	13.03 ± 1.10^a	9.40 ± 0.65^b	8.80 ± 0.38^b
DGLA content (% in DCW)	0.44 ± 0.01^d	0.80 ± 0.02^b	1.02 ± 0.02^a	0.62 ± 0.01^c	0.55 ± 0.02^c
GLA content (% in DCW)	0.48 ± 0.02^d	0.76 ± 0.02^c	1.30 ± 0.05^a	0.94 ± 0.03^b	0.88 ± 0.03^b
Lipid content (% in DCW)	4.50 ± 0.12^c	6.35 ± 0.15^a	6.51 ± 0.34^a	4.92 ± 0.14^b	4.55 ± 0.05^b

All data are presented as mean values with SD. Values marked with different superscript letters in the same row are significantly different (p < 0.05).