TABLE 6.

Kinetic parameters estimated by fitting the models to the experimental data of the engineered A. oryzae cultures grown in the NaNO₃-defined and CSH/ML media.

Kinetic models		Parameters	Values
			NaNO3-defined medium	CSH/ML medium
Cell growth (gX/L.h)	$\frac{dX}{dt}={\mathrm{\mu }}_{max}X\left(1-\frac{X}{X_m}\right)$	μmax (h–1)	0.16	0.17
		Xm (g/L)	18.39	13.72
Glucose consumption (gS/L.h)	$\frac{dS}{dt}=-\left[\left(\frac{1}{Y_{X/S}}\right)\left(\frac{dX}{dt}\right)+m_{S}X\right]$	mS (g/g.h)	0.02	0.09
		YX/S (gX/gS)	0.34	0.21
DGLA production (gD/L.h)	$\frac{d{P}_D}{dt}=\left({\mathrm{\alpha }}_D\mathrm{\mu }+{\mathrm{\beta }}_D\right)X-k_{dD}{(1-\frac{S}{S_0})}^{n_D}X$	αD (g/g)	5.26 × 10–2	1.51 × 10–2
		βD (g/g.h)	1.06 × 10–8	2.59 × 10–8
		kdD (g/g.h)	1.38 × 10–4	3.48 × 10–4
		nD	1.32 × 10–7	6.20 × 10–2
Lipid production (gL/L.h)	$\frac{d{P}_L}{dt}=\left({\mathrm{\alpha }}_L\mathrm{\mu }+{\mathrm{\beta }}_L\right)X-k_{dL}{(1-\frac{S}{S_0})}^{n_L}X$	αL (g/g)	3.94 × 10–3	9.70 × 10–2
		βL (g/g.h)	6.06 × 10–8	1.49 × 10–8
		kdL (g/g.h)	6.38 × 10–3	2.66 × 10–3
		nL	3.33 × 10–1	1.17 × 10–7