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. 2020 Nov 18;11:5872. doi: 10.1038/s41467-020-19760-3

Fig. 3. KLF2 regulates central and peripheral metaflammation to resist metabolic disease.

Fig. 3

a K2KO mice on CD have increased microglial activation within the hypothalamus as exhibited by Iba1 proportional area and characteristic morphological changes, representative images shown, n = 5, p = 0.0277, scale bars: low magnification = 200 µm, high magnification = 20 µm. b Western blot on hypothalamic lysates from CD-fed mice demonstrate increased interleukin-1 beta (IL-1β) production in K2KO mice, n = 3, p < 0.0001. c Mac3 staining of adipose tissue from HFD-fed mice reveals K2KO mice have macrophage expansion as indicated by increased crownlike structures, representative image, LysM n = 3, K2KO n = 5, p = 0.0045, scale bar = 100 µm. d Western blot of JNK activity demonstrating that month HFD-fed K2KO mice have elevated JNK phosphorylation, representative blot of n = 2. e Inflammatory gene qPCR from WAT lysate from CD and HFD-fed mice, n = 4, p values: HFD Il1b = 0.029, HFD Tnfa = 0.0126, HFD Ccl2 = 0.0001. f Inflammatory gene qPCR from liver lysate from CD and HFD-fed mice, n = 4, HFD Il1b p = 0.0446 by unpaired, two-tailed Student’s t-test, comparisons marked or indicated in figure legend. Error bars represent SEM. All mice on were fed CD/HFD for 1 month unless otherwise indicated. n represents biologically independent mice throughout entire figure. Source data are provided as a Source Data file.