Table 1.

R₂-dispersion experiments on GLuc.

Residue	R2(50 Hz)-R2(1 kHz) 1/s
V12	6.66
S16	4.84
T21	2.47
D26	2.12
G28	5.60
L37	2.88
A47	2.61
S61	6.93
M69	4.87
K70	2.07
G75	4.78
T79	6.43
T125	2.26
D138	4.46
L140	4.07
T150	4.46
A152	10.85

Differences of effective ¹⁵N transverse relaxation rates at CPMG rates of 50 Hz and 1 kHz are listed only for peaks that are resolved and their rate difference > 2 1/s. Residues that contribute to the central cavity were underlined. Larger difference was observed for residues in the C-terminal flexible region (indicated by bold letters) indicative of its structural dynamics. The two-state model analysis of CPMG rates of 50, 100, 150, 200, 250, 300, 400, 500, 600, 800, 1000 Hz showed that the estimated exchange rate was 2500 ± 800 1/s. Because the residues showing R2 dispersion spread around several blocks, we judged farther residue-specific analysis using single exchange rate is unreliable.