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. 2020 Nov 18;11:5875. doi: 10.1038/s41467-020-19681-1

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© The Author(s) 2020, corrected publication 2021

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Fig. 3 — a (i) Extracted ion chromatograms (EIC) for the compound with the mass 319.08 Da in reaction mixtures containing malonyl-CoA as substrate and chalcone synthase-like (CHS-L9) (STO07G228250), chalcone synthase (CHS) (STO03G058250), or heat-denatured CHS-L9. (ii) EIC for the mass 335.13 Da in reaction mixtures containing ¹³C₃-malonyl-CoA containing CHS-L9 or heat-denatured CHS-L9 enzyme. Dots in the structure represent ¹³C-labeled carbons. b (i) EIC for the mass 301.07 Da in reaction mixtures containing malonyl-CoA as substrate containing CHS-L9, CHS, or heat-denatured CHS-L9. The inset shows a zoomed region of the EIC chromatogram. (ii) EIC for the mass 317.12 Da in reaction mixtures containing ¹³C₃-malonyl-CoA containing CHS-L9 or heat-denatured CHS-L9 enzyme. c PKS-mediated biosynthetic pathway of anthraquinones. Two pathway intermediates, atrochrysone carboxylic acid and endocrocin anthrone, were produced in the CHS-L9-catalyzed reaction mixture.