Figure 4.

Gene set enrichment analysis of single-nuclei transcriptomes. Circos plots of functional enrichment analysis for each cell type indicates marker functions (adjusted P ≤ .05 compared with other cell types; inner ring; green) and functions showing increased (red) or decreased (blue) enrichment scores following TCDD treatment (outer ring). Enrichment scores for 6513 gene sets were determined using Gene Set Variation Analysis, grouped based on gene membership similarity using Enrichment Map, and summarized using AutoAnnotate, followed by manual curation, as described in the Materials and Methods. Asterisks indicate functions discussed in the manuscript.