Figure 2.

Endotoxemia decreases hepatic transcription and protein content of selenoprotein P. Adult male mice were exposed to sublethal LPS (5 mg/kg, IP) and hepatic whole organ homogenate was evaluated. (A) Selenop mRNA expression at 0, 2, 4, 8, and 24 h after LPS, (B) Representative Western blots of hepatic SELENOP evaluated 0, 8 and 24 h after LPS exposure, (C) Densitometric analysis of decrease in SELENOP protein content expression evaluated 0, 8, and 24 h after LPS exposure. Results are normalized to calnexin loading control, and expressed as a ratio to unexposed control mice. N = 4-7 for all groups. Data are presented as mean ( ± SEM), *p < 0.05 vs control, by one way ANOVA.