Figure 3.

Endotoxemia decreases transcription and protein content of hepatic selenoenzymes. Adult male mice were exposed to sublethal LPS (5 mg/kg, IP) and hepatic whole organ homogenate was evaluated at 0, 8, and 24 h. Fold changes in mRNA expression are shown normalized to unexposed samples. (A) Gpx1 mRNA expression, (B) Gpx4 mRNA expression, (C) TrxR1 mRNA expression, and (D) TrxR2 mRNA expression. (E) Representative Western blots of hepatic Gpx1, Gpx4, TrxR1 and TrxR2 after LPS exposure. Densitometric analysis of decrease in (F) Gpx1, (G) Gpx4, (H) TrxR1, and (I) TrxR2 protein content expression. Results are normalized to calnexin loading control, and expressed as a ratio to unexposed control mice. N = 4–6 for all groups. Data are presented as mean ( ± SEM), *p < 0.05 vs control, by one way ANOVA or two sided t-test.