Fig. 1.
Ehmt1 haploinsufficiency delays PV and PNN expression in the auditory cortex during the auditory critical period. a Primary auditory cortex, layers 2/3 and 4 are marked in red. b Overview of PV+ (green) and PNN+ (red) development in the auditory cortex over time. Layer numbers are marked on the left. c Neurons stained for Parvalbumin (green) and PNNs (red) in the primary auditory cortex, layer 2/3. d Quantification of the density of PV+, PNN+, and PV/PNN double-labelled cells. N P14 = 63/38, P28 = 40/39, P56 = 22/24 images, from 1 to 4 slices per animal, P14 = 6/4, P28 = 3/3, P56 = 3/3 Ehmt1+/+/ Ehmt1+/− animals. e Neurons stained for Parvalbumin (green) and PNNs (red) in the primary auditory cortex, layer 4. f Quantification of the density of PV+, PNN+, and PV/PNN double-labelled cells. N P14 = 66/38, P28 = 40/37, P56 = 13/13 images, from 1 to 4 slices per animal, P14 = 6/4, P28 = 3/3, P56 = 3/3 Ehmt1+/+/ Ehmt1+/− animals.
Source for a Allen Adult Mouse Brain Atlas, https://atlas.brain-map.org/atlas?atlas=1. Image Credit: Allen Institute. Scale bars in b 500 µm, c, e 20 µm. Note that the intensities in c, e are matched within a timepoint, but not across timepoints. Scale in d, f cells/mm2, Dot plot with mean ± SEM, color-coded per mouse. *p < 0.05, **p < 0.01; Nested ANOVA with mice as subgroups. See Supplementary Table 1 for exact values