Figure 3.

Ectopic miR-1260b expression decreased the chemosensitivity of A549 and H1299 cells to paclitaxel or docetaxel. (A) Expression levels of miR-1260b in A549 and H1299 cells transfected with mimic NC or miR-1260b mimic were determined by qRT-PCR assay. IC₅₀ values for paclitaxel or docetaxel in A549 (B) and H1299 (C) cells transfected with mimic NC or miR-1260b mimic as determined by MTT assays. (D) Colony formation assays revealed proliferation ability of A549 (upper) and H1299 (lower) cells transfected with mimic NC or miR-1260b mimic following treatment of paclitaxel (20 μg/L) or docetaxel (5 μg/L), respectively. (E) Flow cytometry indicated cell cycle of A549 (upper) and H1299 (lower) cells transfected with mimic NC or miR-1260b mimic following treatment of paclitaxel (20 μg/L) or docetaxel (5 μg/L), respectively. Paired t-test was applied to compare the differences between two groups. Similar results were obtained in 3 independent experiments and are shown as mean ± SD. *p < 0.05; **p < 0.01 compared with NC group.