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. 2020 Nov 5;11:560696. doi: 10.3389/fpls.2020.560696

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Copyright © 2020 Li, Liu, Li, Wang, Zheng and Hou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The Arabidopsis UGT76C2 glycosylates cytokinin in transgenic rice. (A) RT-PCR analysis of the mRNA levels of UGT76C2 in transgenic rice plants. (B) Trans-zeatin catalyzing activity of the total proteins extracted from WT (1), OE2 (2), OE41 (3), and analysis of total glycosides extracted from WT (4), OE2 (5) and OE41 (6) after 150 n mol trans-zeatin treatment for 24 h. The product (a) indicates trans-zeatin-7-N-Glc, (b) indicates trans-zeatin-9-N-glc. The experiments were conducted in three biological replicates. (C) LC-MS confirmation trans-zeatin-7-N-Glc and trans-zeatin-9-N-glc. (D) The content of cytokinin in 2-week-old seedlings of WT and UGT76C2 transgenic rice. Bars show standard deviations of three biological replicates. Asterisks indicate significant differences between the wild type and the overexpression lines evaluated with Student’s t-test (^∗P < 0.05).