FIGURE 6.

Quantitation of spxB expression and hydrogen peroxide in ΔssaACB fermentor cultures. (A) Expression of the spxB gene in fermentor-grown ΔssaACB cells as determined by RNA-seq analysis. Average TPM at T_–20 and log₂ fold change values for each after Mn depletion time point are displayed. TPM values greater than 1,000 are full saturation (green). Positive log₂ fold change values (red) are genes upregulated in after Mn depletion samples as compared to T_–20, while negative values (blue) indicate downregulated genes. Values in bold indicate significant changes in expression by adjusted P-value (≤0.05). (B) H₂O₂ levels of the BHI culture supernatant were measured at each time point. Means and standard deviations of at least four replicates are shown. Significance was determined by one-way ANOVA with a Tukey-Kramer multiple comparisons test, comparing each post-EDTA time point to T_–20. **P ≤ 0.01, ***P ≤ 0.001.