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. 2020 Nov 5;8:584232. doi: 10.3389/fcell.2020.584232

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Copyright © 2020 Adamo, Delfino, Gatti, Bonato, Takam Kamga, Bazzoni, Ugel, Mercuri, Caligola and Krampera.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunological characterization of HS-5 and HS-27A cell lines. (A) FACS immunophenotypic analysis of primary MSCs, HS-5, and HS-27A cell lines showing the expression profile of CD54, CD106, CD274, HLA-ABC, and HLA-DR in resting or primed condition. Data are represented as mean of gMFI (geometric mean fluorescence intensity) normalized on FMO (fluorescence minus one) control ± SEM. (B–D) Relative PBMCs proliferation following 4 days of co-culture with resting or primed MSCs, HS-5 or HS-27A, respectively. PBMCs proliferation was calculated on living CD45⁺ cells according to CFSE dilution method by measuring CFSE gMFI and normalized on activated PBMCs cultured in absence of stromal cells. Data are represented as mean ± SEM. (E) Representative proliferation peaks of living CFSE⁺CD45⁺ PBMCs following the co-culture with resting or primed MSCs (gray), HS-5 (green), and HS-27A (dark red).