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HS-5 cell line immunosuppressive activity on T, B, and NK proliferation in resting and primed condition. (A,B) Relative T, B, and NK proliferation following the co-culture with resting or primed MSCs (gray) and HS-5 (green), respectively. Cell proliferation was calculated on living CD45+ cells according to CFSE dilution method by measuring CFSE gMFI and normalized on activated IECs cultured in absence of stromal cells. Data are represented as mean ± SEM.
