Fig. 1.

mRNA and protein expression of the APOBEC3 family in response to irradiation. (A) After a 10 Gy X-ray dose, the cells were incubated for 3, 6 or 24 h, and then total RNA was extracted. Real-time RT-PCR was performed using primers to amplify APOBEC3B, APOBEC3C, APOBEC3D, APOBEC3F and APOBEC3G. The mRNA expression levels at each time point were calculated relative to those of unirradiated HepG2 cells. (B) Cells were irradiated with 2 or 10 Gy X-ray (1 Gy/min); incubated for 0, 24, 48 or 72 h; and then lysed to perform immunoblotting for APOBEC3B, APOBEC3C, APOBEC3D, APOBEC3F, APOBEC3G and actin.