Table 2.

Sequence analysis of HPRT point mutants. The mutated position in the exon ±2 bp is indicated and numbered according to its cDNA position. Affected bases and the 5′ and 3′ four bases flanking each side are shown

Cells	IR dose	Mutant	Position of mutation	Target sequencea 5′ to 3′			Type of mutation	Amino acid change
HepG2	0 Gy	H 8–2, H 8–3, H 8–5	c.143	GAAC	G	TCTT	GC > TA	48 R > H
		H 8–1	c.551	AATT	C	CAGA	GC > TA	184 P > Q
		H 2–3, H2–6	c.610–1	tata	g	CATG	GC > AT
		H8–6	c.623	GTCA	T	TAGT	AT > CG	208 I > S
		H 5–1	None
	2 Gy	H2 5–9	c.114	TTCC	T	CATG	AT > GC
			c.542	GGAT	T	TGAA	AT > TA	181 Y > F
			c.612	agCA	T	GTTT	AT > GC
		H2 7–8	c.220	TAAA	T	TCTT	AT > GC	74 F > L
			c.346	AAAA	G	TAAT	GC > AT	116 V > I
			c.385–1	tcta	g	AATG	GC > TA
		H2 7–9	c.302	ATCA	G	ACTG	GC > AT	101 R > K
		H2 6–20	c.402 + 1	GGAA	g	TAAT	GC > TA
		H2 5–15	c.532 + 2	ACTg	t	aagt	AT > TA
		H2 5–17	None
Mock	0 Gy	C 8–1, C 8–2, C 8–6, C 8–7	c.238	GCTG	G	ATTA	GC > TA	80 D > Y
		C 3–27	c.580	CCTT	G	ACTA	GC > AT	194 D > N
		C 8–8	None
	2 Gy	C2 4–7	c.95	GATT	T	GGAA	AT > GC	32 L > S
		C2 5–15	c.532 + 2	ACTg	t	aagt	AT > TA
		C2 4–6	None
A3B	0 Gy	G-3-1	c.59	CTTG	A	TTTA	AT > TA	20 D > V
		G 6–8	c.111	TTTA	T	TCCT	AT > CG	37 I > M
		G 3–8	c.319–1	acta	g	AATG	GC > CG
		G 3–18	c.319–1	acta	g	AATG	GC > AT
			c.346	AAAA	G	TAAT	GC > AT	116 V > I
		G 3–15	c.385–1	tcta	g	AATG	GC > CG
			c.543	GATT	T	GAAA	AT > GC
		G 4–3	c.403	aaag	G	ATAT	GC > AT	135 D > N
		G-3-2	c.520	TGGA	T	ATAA	AT > CG	174 Y > D
	2 Gy	G2 5–35	c.165	TGAA	G	GAGA	GC > CG	55 K > N
			c.188	ATTG	T	AGCC	AT > CG	63 V > G
		G2 5–10	c.166	GAAG	G	AGAT	GC > TA	56 E > *
		G2 5–23	c.229	TGCT	G	ACCT	GC > TA	77 D > Y
		G2 4–3	c.384 + 1	AAAG	g	tatg	GC > AT
		G2 4–1	c.428	ACAA	T	GCAG	AT > TA	143 M > K
A3B KO	0 Gy	3B 5–1	c.28–2	tttc	a	gATT	AT > TA
	2 Gy	3B2 5–2	c.385–2	ttct	a	gAAT	AT > TA
		3B2 5–28	c.402 + 1	GGAA	g	taag	GC > CG

^aIntron sequences are shown in lower case letters, whereas upper case indicates exon sequence.

^*An asterisk indicates a terminal codon.