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. 2020 Nov 5;7:575938. doi: 10.3389/fvets.2020.575938

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Copyright © 2020 Chow, McGrath, de Arruda Saldanha, Whalen, Packer and Dow.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescence characterization and teratogenicity of NPC. NPCs immunostained for detection of intracellular neural stem cell specific antigens, including nestin (A), vimentin (B), and beta-tubulin (C), and pluripotency factors Oct3/4 (E) and Nanog (F). Bright field image showing cluster of neural stem cells formed in vitro (D). Inset boxes depict staining with corresponding isotype antibodies. Dissociated NPCs were embedded in Matrigel and injected s.c in NOD/SCID mice. Teratomas failed to form in any of the 4 mice injected with NPCs (G) and tumor tissues was also not detected after subcutaneous dissection (H).