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Lineage specific differentiation scheme of NPC. Bright field images (10x) of NPC cultures (A) showing spheroidal clusters in suspension, as well as differentiated neurons (C) with elongated thin projections adhered to culture dish. Astrocytes (B) with star shaped glial morphology, and oligodendrocytes (D) with branching microglia. Schematic of the cell culture differentiation protocol, including growth factors and substrates used as well as differentiation time (E).
