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. 2020 Nov 5;7:575938. doi: 10.3389/fvets.2020.575938

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Copyright © 2020 Chow, McGrath, de Arruda Saldanha, Whalen, Packer and Dow.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of terminally differentiated neural cells. Differentiated neurons expressed lineage specific markers MAP2a (A), vimentin (B), and TUJ1 (C). Astrocytes expressed GFAP (D). Inset boxes depict staining with appropriate isotype control antibodies. NPC cultures exhibited upregulated SOX2, Kit, and NXK6.1 compared to skin fibroblasts derived from the same animal (E). Fold changes shown in log scale on y-axis. Astrocyte cultures upregulated mRNA for GFAP, NGF, and RASSF10 (G). Mature neuron cultures upregulated NeuN, MAP2, and NeuroD6 (F), while oligodendrocyte cultures upregulated OLIG2, OMG, and MBP (H).