Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 5;7:597843. doi: 10.3389/fvets.2020.597843

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Zhang, Zhang, Zheng, Liu, Jiang, Du, Li, Li, Yu, Zhou, Tong, Zhao, Tong and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Expression of His-tagged CD163 SRCR5-6 protein and its purification with SDS-PAGE. (A) Amplification of CD163 nucleotides 151-3348 and CD163 SRCR5-6. (B) Purification of pCold-I-SRCR5-6 with SDS-PAGE. Lanes a-c contain the supernatants of cells expressing pCold-I, pCold-I-SRCR5-6, and pCold-I-CD163, respectively, induced with IPTG, after ultrasonication. Lanes d-f contain the pellets of cells expressing pCold-I, pCold-I-SRCR5-6, and pCold-I-CD163, respectively, induced with IPTG, after ultrasonication. (C) Analysis of purified of His-tagged CD163 SRCR5-6 protein with SDS-PAGE. (D) Identification of purified His-tagged CD163 SRCR5-6 protein with western blotting.