Figure 3.

Mapping the epitope of mAb 1D7. (A) A series of truncated SRCR5-6 were constructed to pCold-TF and was successfully expressed in E. coli BL21(DE3) cells. Protein expression was then induced with IPTG. After incubation at 16°C for 12 h, the cells were harvested for SDS-PAGE. Schematic diagram and western blotting results using 1D7 to probe truncated TF-SRCR5-6. (B) A battery of peptides corresponding to amino acids 497–506 were synthesized. Four hundred nano grams peptides were coated to the ELISA plates in order. Indirect ELISA was used to analysis the epitope of 1D7.