Figure 4.

Role of CD163 antibody 1D7 in blocking PRRSV infection. (A) Porcine AMs were pre-treated with different doses of 1D7 supernatant before PRRSV infection for 2 h at 37°C. The cells were then infected with HP-PRRSV HuN4, and incubated for 2 h at 37°C. The total RNA of AMs were extracted, and RT-qPCR was used to detect the transcription level of ORF7 mRNA. (B) Porcine AMs were pre-treated with different doses of 1D7 ascites fluid for 2 h at 37°C followed by HuN4-EGFP infection at a MOI of 0.005 for 48h at 37°C; the cell supernatants were then collected, and the total RNA of supernatants were extracted according to M&M. RT-qPCR was used to identify viral replication. (C) TCID₅₀ was also used to identify viral replication. Error bar: mean ± SEM; **p ≤ 0.01; ***p ≤ 0.001.