Table 1.
Primer for plasmids construction.
| Primera | Sequence (5′-3′)b |
|---|---|
| pColdI-477-682aa-F | TCGGTACCCTCGAGGGAATGCCCAGGCTGGTTGGAGGGGAC |
| pColdI-477-682aa-R | AGCTTGAATTCGGATCTCATGAGCAGATTACAGAGGCCAC |
| pColdI-51-1116aa-F | CTCGAGGGATCCGAAATGCTGAGGCTAACGGGTGGTG |
| pColdI-51-1116aa-R | GTCGACAAGCTTGAATTTCATTGTACTTCAGAG |
| pCold-TF-477-545aa-F | AGGCATATGGAGCTCGGTACCCCCAGGCTGGTTGGAGGG |
| pCold-TF-477-545aa-R | TTCGGATCCCTCGAGGGTACCTCACTGGAATTCTTCAGCCCAGATC |
| pCold-TF-535-614aa-F | AGGCATATGGAGCTCGGTACCAGTGGACAGATCTGGGCTGAAG |
| pCold-TF-535-614aa-R | TTCGGATCCCTCGAGGGTACCTCATTCCATGTCCCAGTGAGAGTTGC |
| pCold-TF-604-682aa-F | AGGCATATGGAGCTCGGTACCTCCCTCTGCAACTCTCACTGGG |
| pCold-TF-604-682aa-R | TTCGGATCCCTCGAGGGTACCTCATGAGCAGATTACAGAGGCCACTT |
| pCold-TF-477-506aa-F | AGGCATATGGAGCTCGGTACCCCCAGGCTGGTTGGAGGG |
| pCold-TF-477-506aa-R | TTCGGATCCCTCGAGGGTACCTCAGAAGTCAGAATCACAGACGGTGC |
| pCold-TF-487-516aa-F | AGGCATATGGAGCTCGGTACCTCTGGTCGTGTTGAAGTACAACATG |
| pCold-TF-487-516aa-R | TTCGGATCCCTCGAGGGTACCTCACCTGCACAGCACGCTGGC |
| pCold-TF-497-526aa-F | AGGCATATGGAGCTCGGTACCACGTGGGGCACCGTCTGT |
| pCold-TF-497-526aa-R | TTCGGATCCCTCGAGGGTACCTCAGAGGGAAACCACAGTGCCG |
| pCold-TF-507-536-aa-F | AGGCATATGGAGCTCGGTACCTCTCTGGAGGCGGCCAGC |
| pCold-TF-507-536-aa-R | TTCGGATCCCTCGAGGGTACCTCAACTTCCTTCTCCAAAGTGAGCTCC |
a
F denotes forward PCR primer; R denotes reverse PCR primer.
b
Restriction sites, and homologous arm are underlined.