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. 2020 Nov 5;8:587691. doi: 10.3389/fcell.2020.587691

FIGURE 4.

FIGURE 4

GLI2 dot disappears from the mother centriole in CEP164-mutated Panc1 cells. (A–C) The indicated cells were cultured in serum-fed medium for 48 h and immunostained with anti-glutamylated tubulin (green) and anti-GLI2 (red) antibodies. (A) DNA was stained with Hoechst (blue). Scale bar, 2.5 μm. (B) The percentage of cells with GLI2 dot at the non-ciliated centrosome was determined. The average of three independent experiments is shown; >100 cells were scored each time. (C) The quantified fluorescence intensity of GLI2 at centrosome is shown. n = 31 (WT + EV), 25 (Cep164-1 + EV), 35 (Cep164-1 + Cep164). (D) Panc1 cells were cultured in serum-fed medium for 48 h and immunostained with anti-CP110 (red), anti-CEP164 (blue), and anti-GLI2 (green) antibodies. Two representative images are shown. Scale bar, 2.5 μm. (B,C) All data are shown as mean ± SEM. **p < 0.01 compared with Cep164-1 + EV (B) (Chi-squared test), compared with Cep164-1 + EV (C) (two-tailed Student’s t-test).