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. 2020 Nov 5;14:595783. doi: 10.3389/fncir.2020.595783

Figure 2.

Figure 2

ARC POMC neurons project to ER-α-expressing cells in the MeA. (A) Schematic illustration of our experimental configurations. AAV1-FLEX-GFPsm was stereotaxically injected into the ARC of the POMC-Cre mice. (B) Images of fluorescence confocal microscopy showing that ARC POMC neurons send projections to ER-α-positive cells (top panel). GFP-positive fibers and axonal terminals were observed in the MeA where ER-α-positive cells were located. Scale bar: 100 μm. Bottom panel: images on the expanded scale (white square area in the top panel). (C) Images of fluorescence confocal microscopy showing that ER-α-positive cells receive POMC input from the ARC. GFP-positive fibers and axonal terminals made synaptic contacts with a subset of ER-α-positive cells (white arrowheads). Scale bar: 10 μm. (D) Images of fluorescence confocal microscopy showing co-expression of ER-α and MC4Rs (white arrows). Scale bar: 10 μm.