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. 2020 Nov 5;7:546445. doi: 10.3389/fmed.2020.546445

Figure 1.

Figure 1

Adiponectin deficiency aggravates liver injury and steatosis as well as sensitizes to HFD-induced NLRP3 inflammasome activation. Four-week-old male wild type (WT) and adiponectin-knockout (APN KO) mice were fed either ND or HFD for 12 weeks. Liver tissue and serum samples were harvested at baseline, 4w, 8w, and 12w, respectively. (A) The body weight and the levels of adiponectin after HFD (B) the serum levels of ALT, AST, and the concentrations of TG and CHOL were determined by colorimetric assay at baseline, 4w, 8w, and 12w. (C) H&E staining and oil red staining in liver tissue sections for evaluation of inflammation and steatosis at different stages, the semi-quantitative analysis of ORO staining was performed by image J. (D) NLRP3, ASC, Capase1, IL1β, IL18, TNFα, and IL6 mRNA expression in liver tissue were detected by qPCR. (E) NLRP3, caspase1, and IL1β immunohistochemical staining in liver tissue sections from mice-fed with HFD at 12w. Results was presented as mean ± SEM, n = 6 mice per group. Differences between two groups were compared using a Student's t-tests. Differences between multiple groups were compared using one-way analysis of variance. ***p < 0.001, **p < 0.01.