Figure 2.

The effects of adiponectin deficiency on ROS production and NFκB/AMPK/MAPK signaling pathways. (A) The ROS levels in liver tissue (B) NFκB and (C–E) AMPK/ p-AMPK, JNK/ p-JNK and ErK1/2/p-ErK1/2 protein expression was detected by quantitative analysis of WB, quantitative data were normalized by actin. The same image for actin has been reused in this figure because the loading control and involved protein was from the same batch of samples, in the case of loading equivalent amounts of total protein, actin were only detected once on a separated membrane to observe whether the internal parameter bands are consistent among the samples. Results was presented as mean ± SEM, n = 6 mice per group, Differences between two groups were compared using a Student's t-tests. Differences between multiple groups were compared using one-way analysis of variance ^**p < 0.01, ^*p < 0.05.