Figure 6.

Adiponectin plays a protective role against PA-mediated NLRP3 inflammasome activation via AMPK-JNK/Erk1/2-NFκB/ROS signaling pathways. Hepatocytes were pretreated with APN 2 h prior to incubation with PA for 24 h. For AMPK inhibition experiment, Compound C (10 μM) were using 2 h prior to adiponectin. (A) Western blot images and quantified levels of (B–E) total AMPK and phosphorylated AMPK, total JNK, and phosphorylated-JNK, total ErK1/2 and phosphorylated-ErK1/2, total NFκB p65, and phosphorylated-p65. Quantitative data were normalized by actin. (F) The ROS production in hepatocytes was detected by BODIPY fluorescence dye. Results was presented as mean ± SEM, all experiments were performed at least three times and at least in triplicate. Differences between two groups were compared using a Student's t-tests. Differences between multiple groups were compared using one-way analysis of variance. ^**p < 0.01, ^*p < 0.05.