Skip to main content
. 2020 Nov 5;107(5):963–976. doi: 10.1016/j.ajhg.2020.10.002

Figure 4.

Figure 4

Disruption of Nckap1 Promotes Neuronal Migration in Mouse Embryonic Cortical Development

(A) Wild-type mouse (B6) brains were electroporated at E13.5 with Nckap1-shRNA and collected at E17.5. More GFP-positive neurons appear in the cortical plate (CP), while fewer fluorescent cells are located at the intermediate zone (IZ) and ventricular/subventricular zones (VZ/SVZ) in Nckap1-shRNA transferred brains compared to WT controls.

(B) Reducing the interval time for harvesting (E14.5–E16.5), we find more positive cells at IZ in Nckap1-shRNA transferred brains compared to WT controls. The statistical results for the proportion of GFP-positive neurons in CP, IZ, and VZ/SVZ are shown. The bar represents standard deviation. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Scale bar = 100 μm.