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. 2020 Oct 23;107(5):911–931. doi: 10.1016/j.ajhg.2020.10.001

Figure 2.

Figure 2

Effects of Apigenin Treatment on Global Gene Expression In Vitro and In Vivo

(A) Principal-component analysis (PCA) of DEX gene expression in untreated and apigenin-treated T21 (n = 7) and sex- and age-matched euploid amniocytes (n = 7). Apigenin treatment induced subtle gene expression changes in T21 and euploid amniocytes. Solid lines link each untreated cell line to its treated counterpart.

(B) PCA of DEX gene expression in untreated and apigenin-treated Ts1Cje (n = 5) and wild-type (WT) (n = 5) E15.5 embryonic forebrain. Apigenin induced significant changes in gene expression in both Ts1Cje and WT embryos.

(C) Heatmap demonstrating the effect of apigenin on DEX genes in Ts1Cje versus WT embryos. WTPow, untreated WT; Ts1CjePow, untreated Ts1Cje; WTAPIG, apigenin-treated WT; Ts1CjeAPIG, apigenin-treated Ts1Cje.

(D) Apigenin partially rescues the expression of several Mmu16 trisomic genes, including Kcnje, Il10rb, Ets, Ifngr2, Pcp4, Dscam, Agtpbp1, Dtna, and Cav1.