. 2020 Oct 23;107(5):911–931. doi: 10.1016/j.ajhg.2020.10.001

Table 3.

Effects of Apigenin Treatment on Developmental Milestones in Ts1Cje Pups

Milestone	Parameter Measured	WT_Pow (n = 31)	Ts1Cje_Pow (n = 19)	Ts1Cje_Apig (n = 26)	WT_Apig (n = 25)
Surface righting	latency delay significant at postnatal day versus WT		P6^∗, P7^∗∗, P8^∗∗, P9^∗∗	P7^∗, P8^∗∗, P9^∗	P7^∗, P8^∗∗, P9^∗
Surface righting	milestone day	7.03 ± 1.47	8.29 ± 1.45^∗	7.35 ± 2.11	8.36 ± 2.02^∗
Cliff aversion	latency delay significant at postnatal day versus WT		P5^∗∗, P6^∗∗, P7^∗, P8^∗∗, P9^∗	P5^∗, P6^∗, P7^∗, P8^∗	P5^∗, P6^∗, P7^∗, P8^∗
Cliff aversion	milestone day	5.71 ± 1.62	7.12 ± 2.20^∗	6.46 ± 2.06	6.76 ± 2.83^∗
Negative geotaxis	latency delay significant at postnatal day versus WT		P3^∗, P4^∗∗, P5^∗, P6^∗, P7^∗∗	P4^∗, P5^∗∗, P6^∗, P7^∗∗	P4^∗
Negative geotaxis	milestone day	6.16 ± 2.01	7.17 ± 2.70^∗	7.20 ± 2.27^∗	6.16 ± 1.54
Forelimb grasp	latency delay significant at postnatal day versus WT		P5^∗, P6^∗, P7^∗	P5^∗, P6^∗, P7^∗∗∗, P8^∗	P6^∗, P7^∗∗, P8^∗
Forelimb grasp	milestone day	7.03 ± 1.30	8.12 ± 1.17^∗	8.30 ± 1.33^∗∗	8.00 ± 1.15^∗
Open field	latency delay significant at postnatal day versus WT		P14^∗, P15^∗∗, P16^∗, P17^∗, P18^∗	P14^∗, P15^∗∗, P16^∗, P17^∗, P18^∗, P19^∗, P20^∗, P21^∗	ns
Open field	milestone day	13.54 ± 1.84	13.82 ± 1.42	14.68 ± 1.83^∗	13.32 ± 1.40
Air righting	milestone day	16.48 ± 1.39	18.88 ± 1.67^∗∗∗	18.10 ± 2.02^∗∗	17.00 ± 1.87
Eye opening	milestone day	14.39 ± 0.92	15.60 ± 2.03^∗	14.79 ± 1.36	14.36 ± 0.76
Ear twitch	milestone day	16.00 ± 0.97	17.64 ± 1.27^∗∗∗	17.79 ± 1.68^∗∗∗	16.64 ± 1.25
Auditory startle	milestone day	17.12 ± 1.36	18.00 ± 2.00	17.58 ± 1.89	16.28 ± 1.62

Postnatal days at which significant delays were measured are indicated by the postnatal day (P) and the level of significance [^∗ (p < 0.05), ^∗∗ (p < 0.01) and ^∗∗∗ (p < 0.001)] compared to WT_Pow. The average day at which each developmental milestone is reached (milestone day) is indicated. Significant differences are indicated with the levels of significance. ns, not significant.