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. 2020 Oct 4;7(22):2002747. doi: 10.1002/advs.202002747

Figure 4.

Figure 4

HITTERS promotes OSCC progression and correlates with OSCC clinicopathological features. A) CCK8, B) colony formation test, and C) EdU incorporation test confirmed that knockdown of HITTERS suppressed OSCC proliferation in vitro. D,E) Stably knockdown of HITTERS significantly suppressed tumor volume and tumor weight in D) SCC25 and E) CAL27 subcutaneous xenograft model. F) Wound healing test and G) transwell assay confirmed knockdown of HITTERS suppressed OSCC migration and invasion ability in vitro. Stably knockdown of HITTERS significantly suppressed H) SCC25 and I) CAL27 pulmonary metastasis nodule formation ability in vivo. The green fluorescent protein (GFP) fluorescense imaging of lungs was also presented. J) qPCR analyzed the relative fold of HITTERS in 48 OSCC tissues normalized by their paired adjacent normal tissues. K) qPCR results indicated the expression of HITTERS in OSCC was higher than their paired adjacent normal tissues. Paired t‐test was used. L) Kaplan–Meier curves showed high HITTERS expression had poor over‐all survival. Log‐rank test was used. For (A)–(I) Student t‐test was used. For (A), (D), and (E), Student t‐test was used for each time point. Note: *, P < 0.05; **, P < 0.01, ***, P < 0.001.