Figure 6.

Effect of hypothermia and its subsequent rewarming on the cell death profile of TAMH. Hypothermia, at 32°C (moderate), was performed for 24 h on TAMH during 5 mM AILI followed by a 24 h rewarming back to the physiological temperature at 37°C. At both (A) hypothermic and (B) rewarming state, percentage of total cell death was determined with PI staining. Further analysis on the extent of necrotic and apoptotic cell death was also conducted with Annexin V and PI staining during (C) hypothermia and (D) rewarming. The top right quadrant, in blue, represents the extent of necrotic cell death while the bottom right quadrant, in green, represents the extent of apoptotic cell death. The relative extent of cell death, in percentage, has been indicated in each of the quadrants. Data are presented as mean ± SD (n = 3), where unpaired t-test is used to compare the effect of hypothermia or subsequent rewarming on TAMH against their respective controls treated with the same APAP dose. **p < 0.01.