Figure 3.

Effects of gut microbiota on differentiation‐related factors in osteoblasts and osteoclasts. A, The effect of supernatant of LABS and LGG, and sodium butyrate on ALP activity and OCN concentration in osteoblasts MC3T3‐E1. B, The effect of supernatant of LABS and LGG, and sodium butyrate on the relative promoter activity of AP‐1 in osteoblasts MC3T3‐E1 and osteoclasts RAW 264.7. C, The effect of supernatant of LABS and LGG, and sodium butyrate on the expression of osteoblast and osteoclast‐related gene in osteoblasts MC3T3‐E1 and osteoclasts RAW 264.7. ^*, P < .05 compared with control group; ^#, P < .05 compared with LABS group; ^△, P < .05 compared with LGG group