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. 2020 Aug 11;34(11):e23471. doi: 10.1002/jcla.23471

Figure 4.

Figure 4

Effects of gut microbiota on the maturity of osteoblasts and osteoclasts. A, The effect of supernatant of LABS and LGG, and sodium butyrate on dark staining of the osteoblasts MC3T3‐E1 cell membrane and cytoplasm after ALP/Alizarin Red staining. B, The effect of supernatant of LABS and LGG, and sodium butyrate on the cytoplasmic volume and additional nuclei in osteoclasts RAW 264.7 after TRAP staining. C, The graph of gut microbiota on the ALP, Alizarin Red, and TRAP staining in osteoblasts MC3T3‐E1 and osteoclasts RAW 264.7 cell. *, P < .05 compared with induction group