Figure 4.

miR‐1225 targets p65. A, Graphical representation of the conserved miR‐1225 binding motif at the 3′‐UTR of NFκB p65. B, Luciferase function exhibited via luciferase reporter constructs carrying either the wild type (WT) or mutated (MU) human p65 3′‐UTR subsequent to miR‐1225 mimic transfection. Luciferase function was normalized to the function of Renilla luciferase. C, p65 expression in samples acquired from HCC patients (n = 10) vs. normal healthy tissue (n = 10) was examined by qPCR. D, Western blotting and E, qPCR were carried out in order to examine p65 expression in Huh‐7, HepG2, and normal liver cells. F, G, Western blotting and H, I, qPCR were conducted to assess p65 protein expression and transcription in HCC cells, respectively, subsequent to transfection with the miR‐1225 mimic and NC mimic. Outcome is displayed as mean ± SD. *P < .05, ***P < .001 vs control group