Figure 5.

NFκB p65 is involved in miR‐1225‐inhibited proliferation, invasion, and migration of HepG2 and Huh‐7 cells. A, B, HepG2 and Huh‐7 cells underwent co‐transfection with of pCDNA3‐p65/pCDNA3 and the miR‐1225 mimic to upregulate the p65 expression. A, C, qPCR and B, D, WB were performed to detect the p65 protein expression in both HCC cell lines. E, F, Proliferation of Huh‐7 and HepG2 cells was measured at 12, 24, 36, 48, 60, and 72 hours after transfection, using the MTT assay. G, H, Invasion and I, J, migration of HepG2 and Huh‐7 cells was examined using the Transwell migration test and wound healing assay. Outcome is displayed as mean ± SD. *P < .05 vs control group