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. 2020 Nov 18;10:20044. doi: 10.1038/s41598-020-76960-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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UBR5 is a modulator of MYC stability. (A) Gene enrichment score in high GFP/mCHERRY-sorted cells relative to unsorted cell population on day 7 of library infection visualized as LogFC. (B) sgRNA-mediated knockout of UBR5 (sgUBR5), FBXW7 (sgFBXW7) or a non-targeting sgRNA control (sgNT) in B6 clone and analysis of GFP, mCHERRY, and GFP/mCHERRY ratio using FACS 7 days post-knockout. (C) MYC degradation screen using an arrayed sgRNA sublibrary. MYC-GFP/mCHERRY cells were infected with lentiviruses encoding for sgRNAs targeting HECT E3 ligases. 2 sgRNAs were used per gene (indicated as 1, 2). Seven days after infection and selection with puyromycin, GFP/mCHERRY was measured using flow cytometry and the increase in GFP/mCHERRY ratio upon knockout of the singlegenes was compared to a non-targeting control (sgNT).