Table 3.
List of significant gene ontology terms associated with biological processes, molecular functions, and cellular components related to fertility identified by NetworkAnalyst using the protein–protein interaction network built by the prioritized candidate genes for scrotal circumference and sperm motility and the directly connected nodes.
| GO category | GO term | FDRa-corrected p value | Gene names |
|---|---|---|---|
| Biological process | Regulation of MAPK cascade | 8.13 × 10–1 | TCP1*, PIK3R1 |
| Spermatid differentiation | 1 | PRLR* | |
| Regulation of hormone secretion | 1 | ACACA | |
| Acetyltransferase activity | 8.88 × 10–4 | CDC27, JAK1, GRB2, PIK3R1 | |
| Zinc ion binding | 2.12 × 10–3 | JAK1, PIK3CA, PLK1, CDK1, PI4KA, SGK1, PRKACA | |
| Molecular function | Lipase activity | 2.55 × 10–3 | GRB2, MAP2K1, PLK1, MAP3K1*, PRKACA, FBXO5* |
| Endonuclease activity | 2.55 × 10–3 | PIK3CA, PLK1, CDK1, MAP3K1*, SGK1, PRKACA | |
| Nuclease activity | 4.58 × 10–3 | JAK1, MAP2K1, PLK1, CDK1, MAP3K1*, SGK1, PRKACA | |
| Cation channel activity | 9.51 × 10–3 | PLCZ1, PLCB1, PLCB4* | |
| Kinesin complex | 1.53 × 10–2 | CDC27, PLK1, CDK1 | |
| Cellular component | Spindle microtubule | 1.53 × 10–2 | RAC1, PIK3CA, GRB2, TRAF6, GNA14, MAP2K1, IRAK1, TLR4, TIRAP*, PI4KA, HSP90AA1, XRN1, SGK1, PRKACA, PRLR*, CD40, NPSR1, PPP2CA, RGS17* |
| Cytosol | 4.42 × 10–2 | JAK1, JAK3, TUBB4A, PLK1, CDK1, PPP2CA |
aFalse discovery rate.
*Prioritized candidate gene identified by GUILDify and ToppGene analysis.