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. 2020 Nov 18;11:5885. doi: 10.1038/s41467-020-19619-7

Fig. 3. SARS-CoV-2 virion budding and assembly at the ERGIC membrane.

Fig. 3

Different budding events captured in two tomograms (Supplementary Fig. 6 and Supplementary Movies 2 and 3) of VeroE6 cells infected with SARS-CoV-2 at 16 hpi. A 3D volume rendering is shown for each area with cellular and viral membranes in green and magenta, respectively. S (yellow) and vRNPs (cyan) are represented as subtomogram averages. The S and vRNP locations correspond to the location in the tomogram, vRNP orientations were randomized. A non-local means filter was applied, and 20 slices were averaged. a Overview of budding events at the ERGIC membrane and intracellular released virions inside the ERGIC lumen are indicated. b, c Accumulated S at the lumenal side of the ERGIC membrane. d, e Early virion budding stage with S and vRNPs accumulated at the lumenal and cytosolic ERGIC membrane, respectively. f Assembled viron in the ERGIC lumen in close proximity to the membrane, showing a polarized distribution of S. g Assembled virion further away from the membrane with redistributed S. Scale bars: a 200 nm, b–g 100 nm.