Figure 2.

(a) Transcript levels of vtg gene in fish fed with EE₂ at concentrations of 0 (control), 2.5 and 5 µg/g food for 28 days. (b) Plasma Vtg levels of fish fed 0 (control), 2.5 and 5 µg/g food for 28 days (treatment) and of fish fed 0 (control), 2.5 and 5 µg/g food for 28 days and afterwards a standard diet for 146 and 333 days (recovery). M, male specimens; F, female specimens. *Denote statistically significant differences between treated and control groups and letters indicate differences in the control group at distinct sampling times, as determined by one-way ANOVA and LSD post hoc tests.