Figure 3.

Testis of gilthead seabream fed with 0 (control, a–c), 2.5 (d–i) or 5 (j–l) µg of EE₂/g of food for 28 days showing tubules with spermatogonia stem cells (arrow), primary spermatogonia (solid arrowhead) and cysts of spermatogonia (SG), spermatocytes (SC) and spermatids (SD with plenty of spermatozoa (SZ) in the lumen. Note that besides some interstitial cells (asterisk) and Sertoli cells (open arrowhead), diminished (f,g) or absent (h,i) cysts of proliferating germ cells were found in some individuals fed with 2.5 (g,i) and in those fed 5 µg of EE₂/g of food (j–l). H–E (a,d,f,h,j); PCNA-immunolabelling (b,c,e,g,i,k,l).