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. 2020 Nov 18;10:20067. doi: 10.1038/s41598-020-76902-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Testis of gilthead seabream fed with 0 (control, a,b,e,f), 2.5 (d,g) or 5 (c,h) µg of EE₂/g of food for 28 days followed by a recovery period of 146 days of feeding with a standard diet showing collapsed tubules consisting of mainly spermatogonia stem cells (arrow) and primary spermatogonia (solid arrowhead), expanded interstitial tissue (IT) and necrotic areas (Na). Note the scarce proliferative interstitial (asterisk, f,h), Sertoli (open arrowhead, e,h) and germ (solid arrowhead, g) cells. H–E (a–d); PCNA-immunolabelling (e–h).