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. 2020 Nov 18;10:20067. doi: 10.1038/s41598-020-76902-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Gonads of gilthead seabream fed with 0 (control, a,b) and 2.5 µg of EE₂/g of food for 28 days followed by a recovery period of 333 days of feeding with a standard diet (c). The control fish is a female and has an ovary with secondary oocytes (a) and shrunk testis with collapsed tubules consisting of dark cells and sparse spermatogonia stem cells (b). The treated fish showed testis at spermatogenesis (c) with tubules consisting of with spermatogonia stem cells (arrow), primary spermatogonia (solid arrowhead) and cysts of spermatogonia (SG), spermatocytes (SC) and spermatids (SD) and plenty of spermatozoa (SZ) in the lumen. H–E.