Figure 6.

Viral Escape Mutant Selections with Individual Antibodies and Antibody Cocktails

(A) Results of viral selections with five individual monoclonal antibodies. The number of replicates where escape variants were selected are indicated, color-coded according to whether escape was selected frequently (red) or rarely (white). Mutations present in the RBD of the selected escape variants are indicated.

(B) Each point represents a different amino-acid mutation to the RBD, with the x axis indicating how strongly the mutation ablates antibody binding in our escape maps and the y axis indicating how the mutation affects ACE2 binding (negative values indicate impaired ACE2 binding). All selected mutations were accessible by single-nucleotide changes. The only accessible escape mutation from COV2-2165 that is not deleterious to ACE2 binding is D420Y, but this mutation is highly deleterious for RBD expression (Figure 5B; Figure S5).

(C) Results of viral selections with antibody cocktails, indicating the number of replicates with escape out of the total tested. The data for the single antibodies are repeated from (A). In all panels, antibody names are colored according to where in the RBD the majority of their sites of escape fall: orange for the core RBD, light blue for the RBM, and dark blue for ACE2 contact residues. See also Figure S6.