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In vitro Pce enzyme activity of various pneumococcal strains using p-NPPC as the substrate. The in vitro Pce enzyme activity was defined as the average slope of OD405 over the first 8 h, normalized to the OD600 value of the strain’s working stock. Strains are arranged by increasing Pce activity. Strains with enzyme activity of less than 0.05 units were defined as having no Pce activity for the purpose of this study
