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Time-kinetic analysis of GH50A β-agarase-catalyzed AOS hydrolysates by LC-MS/MS. For hydrolysis of AOS, 1% AOS was incubated with 10 μg/mL enzyme in 20 mM Tris–HCl (pH 7.5) at 35 °C for individual time periods. LC-MS/MS analysis was performed as described in the Experimental Section.
